warning I tried to find a guide but everything I found searching gave me old but also answers of getting good cuttings hard. tried!
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For propagation by hardwood cuttings the cuttings were made in February and March before the beginning of the vegetation period. The cuttings were kept in sand and sawdust until the danger of frost at ground level passed and then they were planted into cold frames.
The cuttings were subjected to treatment with a weak (pink in colour) solution of KMnO4 and with growth regulators, with IBA at 0.005% for a period of 16 hours or with IAA at 0.01% for a period of 5 hours [HROMOVA, 1980]. They were planted into cold frames into two substrates: sand or sand + peat in a ratio of 1:1 and minimum 24% artificial mist. After one year the cuttings were planted into containers or in the open field.
Having in view that by generative propagation some qualities specific to the mother plant appear or disappear and vegetative propagation presents some difficulty, the initiation of in vitro cultures was tested for this species.
The plant material consisted in various explant types: apical meristems, lateral meristems of the 2nd, 3rd, 4th, 5th and 6th degree, fragments of juvenile leaves, shoot fragments from the apical part, shoot fragments with lateral meristem, Fragments of young leaves with veins, ovules, ovary with a fragment from the stem, stem fragment with the apical meristem. For culture initiation nine experimental variants were tested, with MS (1962) basal medium and various concentrations of plant hormones (Tab. 1).
The operations were carried out according to the standard laboratory procedures.”