Skunky Dunk Farms
Cannabinoid Receptor
Ok, I know this can be a can of worms or as they say "down a rabbit hole" but it's a subject that should be available to all of us here on this site especially.
So as just an example, this is a sample of what's required to chase a line.
I recieved this quote from Todd at AG seed co after an inquiry.
It is for the Skunk #1 line.
Skunk #1 is a relatively true breeding variety that is quite unique in the cannabis community.
Robert Clarke describes the breeding that went into Skunk #1 in his research paper titled:
Cannabis Domestication, Breeding History, Present-day Genetic Diversity, and Future Prospects
"A brief summary of the breeding of “Skunk No. 1” will illustrate what is involved in developing a relatively true breeding seed cultivar.
Several plants were grown from a local California “skunk” variety, which was a 2/3 NLD and 1/3 BLD hybrid (e.g., Colombian/Afghan & Mexican) and then all the females were crossed with a single select male. A female identified as skunk plant number one was selected as the highest yielding and most potent, and became the founder of all subsequent generations.
For the following nine growing seasons, at least one branch of each of the (up to 100) female plants was fertilized by pollen from a male selected from the offspring of the previous year’s best female plant. Seeds from the select females were sown the following spring.
After only two or three generations, “Skunk No. 1” was relatively homogenous and true-breeding compared to other hybrid lines that continued to segregate in the F3 and F4 generations, and “Skunk No. 1” was then deemed ready for large-scale selection of parent plants with specific combining ability (SCA) for subsequent breeding.
A total of nearly twenty thousand plants were grown in a common garden from the seeds of ten females from the most promising lines selected from the previous generation, and the ten best females from among all those lines were selected based on vigor, potency, type of effect, flower yield, high flower to leaf ratio, resin gland development, amount of branching and pest resistance, as well as attractive floral aromas and flavors. Ten males were also selected for their vigor, pest resistance, female-type growth form, and aroma.
All 20 selections (male and female) were reproduced asexually to preserve their unique genotypes. Ten clonal copies were made of each of the ten select females. Pollen was collected from each of the ten select male clones, and pollen from a single male was used to fertilize a single copy of each female clone, resulting in 100 individual crosses. Two hundred seeds of each cross were sown (20,000 total) and approximately 100 female progeny of each cross were evaluated for phenotypic consistency (homozygosity) of their favorable agronomic traits (see above). Five female and three male clones were selected and used for “Skunk No. 1” sowing seed multiplication as well as hybrid cultivar development."
So as just an example, this is a sample of what's required to chase a line.
I recieved this quote from Todd at AG seed co after an inquiry.
It is for the Skunk #1 line.
Skunk #1 is a relatively true breeding variety that is quite unique in the cannabis community.
Robert Clarke describes the breeding that went into Skunk #1 in his research paper titled:
Cannabis Domestication, Breeding History, Present-day Genetic Diversity, and Future Prospects
"A brief summary of the breeding of “Skunk No. 1” will illustrate what is involved in developing a relatively true breeding seed cultivar.
Several plants were grown from a local California “skunk” variety, which was a 2/3 NLD and 1/3 BLD hybrid (e.g., Colombian/Afghan & Mexican) and then all the females were crossed with a single select male. A female identified as skunk plant number one was selected as the highest yielding and most potent, and became the founder of all subsequent generations.
For the following nine growing seasons, at least one branch of each of the (up to 100) female plants was fertilized by pollen from a male selected from the offspring of the previous year’s best female plant. Seeds from the select females were sown the following spring.
After only two or three generations, “Skunk No. 1” was relatively homogenous and true-breeding compared to other hybrid lines that continued to segregate in the F3 and F4 generations, and “Skunk No. 1” was then deemed ready for large-scale selection of parent plants with specific combining ability (SCA) for subsequent breeding.
A total of nearly twenty thousand plants were grown in a common garden from the seeds of ten females from the most promising lines selected from the previous generation, and the ten best females from among all those lines were selected based on vigor, potency, type of effect, flower yield, high flower to leaf ratio, resin gland development, amount of branching and pest resistance, as well as attractive floral aromas and flavors. Ten males were also selected for their vigor, pest resistance, female-type growth form, and aroma.
All 20 selections (male and female) were reproduced asexually to preserve their unique genotypes. Ten clonal copies were made of each of the ten select females. Pollen was collected from each of the ten select male clones, and pollen from a single male was used to fertilize a single copy of each female clone, resulting in 100 individual crosses. Two hundred seeds of each cross were sown (20,000 total) and approximately 100 female progeny of each cross were evaluated for phenotypic consistency (homozygosity) of their favorable agronomic traits (see above). Five female and three male clones were selected and used for “Skunk No. 1” sowing seed multiplication as well as hybrid cultivar development."