Thought it would be nice to rejuvenate my library so I just finished the liquid media microclones of my cut library (mostly). I'll be taking some more involved meristem tissue cultures with agar media this week for a handful of some older cuts. I usually go through a whole process to be able to do a full writeup with everything involved but I figured to just post about this stuff as I fumble with it. I'd like to be able to get it done under ideal conditions and successively pare it down until I get the minimum effort/cost with solid success. I used a 25% bleach solution for the tools.
This procedure is a more streamlined tissueponics microcloning that only using a multiplication media to grow out some very small cuts until they can be rooted in a root plug. I got the process from this video:
This procedure is a more streamlined tissueponics microcloning that only using a multiplication media to grow out some very small cuts until they can be rooted in a root plug. I got the process from this video: