The Wang Protocol

[SCJedi]

OK, here is a good starting point.

Each Liter of media should include:
PGR(s) of your choice that is not heat-labile. (Heat-labile PGR's should not be autoclaved or co-autoclaved but rather filter sterilized)
4.43g·L-1 Murashige & Skoog (aka Full MS)
30 g·L-1 Sucrose as a carbon source
6.8 g·L-1 Agar (or another gelling agent)
The pH of the medium adjusted to 5.8
Sterilize in an autoclave at a temperature of 121C and at the pressure of 15 lbs psi for 15 min

Optional:
PPM or another biocide.

 

[Deebs]

This is awesome @SCJedi, the more I read the more interested I get! Where have you found is the best place to get decent PGR materials? Murashige & Skoog primarily. Any preferred brand?

 

[SCJedi]

If you have the scratch go get lab grade materials from fisher or sigma, otherwise us cavemen shop at places like phytotech or caisson labs. I think my last order was from the latter.

You can buy pouches that make enough MS for a single liter of media. I typically buy it 100 grams bottles, that way I know it's always fresh

 

[SCJedi]

I will also add that cavemen use table sugar as a substitute for lab-grade sucrose. It works fine.