The Wang Protocol

SCJedi

In Bloom
OK, here is a good starting point.

Each Liter of media should include:
PGR(s) of your choice that is not heat-labile. (Heat-labile PGR's should not be autoclaved or co-autoclaved but rather filter sterilized)
4.43g·L-1 Murashige & Skoog (aka Full MS)
30 g·L-1 Sucrose as a carbon source
6.8 g·L-1 Agar (or another gelling agent)
The pH of the medium adjusted to 5.8
Sterilize in an autoclave at a temperature of 121C and at the pressure of 15 lbs psi for 15 min

Optional:
PPM or another biocide.
 

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Deebs

The Sentient Naturewalker
Staff member
Moderator
This is awesome @SCJedi, the more I read the more interested I get! Where have you found is the best place to get decent PGR materials? Murashige & Skoog primarily. Any preferred brand?
 

SCJedi

In Bloom
If you have the scratch go get lab grade materials from fisher or sigma, otherwise us cavemen shop at places like phytotech or caisson labs. I think my last order was from the latter.

You can buy pouches that make enough MS for a single liter of media. I typically buy it 100 grams bottles, that way I know it's always fresh
 
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