BH
Tha Dank Hoarder
NEW CANNABIS VIROID- Hop Latent Viroid (HpLVd) with Dr. Tassa Saldi. PhD
Dr. Saldi received her Ph.D. in molecular biology from the University of Colorado in Boulder and completed her post-doctoral studies at the Health Sciences C...
youtu.be
Cannabis Viroid Prevention
30 minute Science LessonJames Loud & Joe Ramahi., PhD. Joe Ramahi is a UCD PhD California Plant Molecular Biologist from the #bayarea 6 years in scaled Canna...
youtu.be
Soil Biology vs. Dudding, Viroids and Pathogens
Soil scientist Scott Skamnes talks to greenhouse expert Eric Brandstad and cannabis cultivator Kevin Jodrey about dudding, hop latent viroid, pathogens, and...
youtu.be
07/26/23
Bryan & Marco S3E26 Understanding HLVd From A Commercial Breeder & Growers
SHOW #2 (Commercial Breeding) Educate Yourself Or Perish…. Hop Latent Virus (HLVd) 👈🏻 What Is It? One study estimates as much as 90% of California cannabis...
www.youtube.com
08/9/23 added video ( very informative)
GrowON Webinar: Dr Punja - Hop Latent Viroid (HpLVd) in cannabis production
Hop Latent viroid (HpLVd) has emerged as a widespread infectious pathogen causing stunt disease on cannabis (Cannabis sativa L.) that has severe economic imp...
youtu.be
08/13/23
woah study is showing over 40% of average seeds have hplvd, this video is very informative and shows some of the best and newest knowledge on this issue.
Understanding and Managing Hop Latent Viroid in Cannabis - Zamir Punja, PhD
Tags: Cannabis Plant Pathogens, Trichomes, HPLVd, Hop Latent Viroid, qPCR Testing, Root Infection, Cannabis Yield, Cannabis DiseaseZamir K. Punja, PhD, prese...
youtu.be
08/21/23
Hop Latent Viroid HLVd Background
Hop Latent Viroid (HLVd) BackgroundProfessor DeBaccoResearch ArticleAdkar-Purushothama, C. R., Sano, T., & Perreault, J. P. (2023). Hop Latent Viroid: A Hidd...
youtu.be
08/22/23
How Can You Prevent Hop Latent Viroid (HLVd) From Spreading?
How Can You Prevent Hop Latent Viroid HLVd From Spreading?Professor DeBaccoAdditional Video Sources:Medicinal Genomics. (2023a, June 8). Understanding and Ma...
youtu.be
08/29/23 = can tissue culture remove hlvd
Can Tissue Culture Remove Hop Latent Viroid (HLVd)
Can Tissue Culture Remove Hop Latent Viroid (HLVd)? Professor DeBaccoTesting/Screening is Still NeededThis is still an important part of the process. By tes...
youtu.be
“OAKLAND, Calif., (March 4, 2019) — Dark Heart Nursery (DHN) proudly announced today that it is the first organization to positively identify hop latent viroid (HpLVd), previously identified as Putative Cannabis Infectious Agent (PCIA), as the cause of “dudding” in cannabis. Since 2018, DHN has also been successfully testing for and eliminating HpLVd through a patent-pending clean plant process.
On the heels of this groundbreaking completed research, the company has also announced that project lead Dr. Jeremy Warren has officially joined DHN as Director of Plant Health.
“The positive identification of the HpLVd pathogen and our patent-pending clean plant process to test for and eliminate it represents a significant advance towards keeping cannabis crops healthy and supporting long-term business growth for cultivators,” said Dan Grace, Founder and President of Dark Heart Nursery. “With Dr. Warren at the helm, we are incredibly excited to now offer diagnostic and curative services for HpLVd to licensed cannabis businesses in California, as well as continue our research in identifying and eradicating additional cannabis-infecting pathogens.”
“Dudding” is a colloquial name for a variety of symptoms, which include loss of vigor, stunting, reduction in yield, reduction in potency and changes in morphology. The syndrome was codified in 2017 by Dr. Rick Crum who first coined the phrase “Putative Cannabis Infectious Agent” (PCIA) to describe it. In 2015, Dr. Crum reported that as many as 35% of observed plants showed PCIA symptoms.
In 2017, Dark Heart Nursery began working with Dr. Warren to determine the cause of PCIA. Symptomatic and asymptomatic plants were collected, and next-generation RNA sequencing was performed to determine a probable cause of the disease. After analysis of the results in November 2017, it was determined that Hop latent viroid (HpLVd) was the most likely candidate pathogen. A genetic test was then developed to aid in differentiating healthy plants from infected plants.
“It is important to note that finding a potential pathogen within a plant was only the first step in determining if the pathogen is indeed causing the disease in question,” said Dr. Warren. “To demonstrate causality, healthy plants were inoculated with an infectious clone of HpLVd to confirm that the developed symptoms were consistent with PCIA. Our team monitored these infected plants, as well as controlled (healthy) plants for three months to track symptom development. After this time, it was apparent that the HpLVd-infected plants were showing symptoms consistent with PCIA, such as yellowing of leaves, malformed leaves, and stunted growth. These results confirmed that HpLVd is causing PCIA symptoms in cannabis.”
In 2014, DHN established the cannabis industry’s first tissue culture laboratory. Among other areas of research, this lab has focused on the development of clean plant protocols through which cannabis can be cured of pathogens and cataloged for later use. Under the leadership of Research Manager Will Roberts, the company developed a patent-pending clean process, which is capable of reliably eliminating HpLVd from infected specimens. For the last year, Dr. Warren’s discovery and subsequent diagnostic tests have allowed DHN’s lab team to reliably and demonstrably eliminate HpLVd. To date, 31 varietals have been cured of the disease.”
Hop Latent Viroid – Dark Heart Nursery
darkheartnursery.com
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What is the Hop Latent Viroid?
The hop latent viroid (HpLVd) is an infectious pathogen that causes disease in cannabis plants. Also known as “dudding” or “dudding disease,” plants infected with HpLVd may or may not show symptoms (symptomatic vs asymptomatic). HpLVd was also previously known as PCIA (putative cannabis infectious agent) until Dark Heart confirmed HpLVd as the cause of PCIA in 2018. HpLVd has been detected throughout the United States and Canada.
HpLVd can be dormant in a plant for extended periods of time before showing symptoms. Symptoms include reduced vigor, abnormal branching, stunted trichome production, reduced potency, and an overall loss of quality and yield.
Why Should Growers Care?
If left untreated, HpLVD can seriously reduce a crop’s potency and yield. Every garden tested in 2019 was infected with HpLVd. Most gardens had an infection rate of about 25-50%, causing an estimated 44 million dollars in losses per year. HpLVd infections typically occur when an infected clone or plant is introduced into a healthy garden, where it spreads to other plants.
How is it spread?
Research about transmission methods is ongoing. The viroid can spread through different types of contact, such as mechanical transmission. This occurs when pruning tools are used on an infected plant, then on an uninfected plant. HpLVd can also be transmitted through the seed of an infected plant, though more research is underway to determine the transmission rate.
The latent nature of HpLVd makes it a challenging pathogen to detect and fully eradicate. A common scenario in an infected garden is that while plants look healthy, they are in fact asymptomatic and don’t exhibit symptoms. As a result, many growers are not aware that the pathogen is present and spreading to other plants in the garden.
What can be done?
The first step is determining if HpLVd is present in a garden. Not all infected plants will “look” infected or show symptoms. A diagnostic screening test is the fastest way to determine the presence of the viroid. If a positive result is found, the infected plant should be removed from the facility immediately and disposed of. Any plants that it was touching should also be quarantined and tested.
If a special or a proprietary strain is fully infected (such as the entire grow or mother block), then eradication via tissue culture is the only way to clear HpLVd from that strain. The tissue culture process utilizes a proprietary treatment that eliminates the viroid and leaves you with a healthy rejuvenated strain.”
“As the name suggests, Hop Latent Viroid (HpLVd) was first identified in hops. Ironically, infected hop plants are asymptomatic. In contrast, infected hemp plants (Cannabis sativa) may or may not manifest any apparent symptoms. Symptoms include stunted growth, reduced vigor, brittle stems with less flower mass and stunted trichomes. This disease is commonly known as dudding.
Viroids are the smallest known pathogens of row crops and fruit trees. Like some plant pathogenic viruses, viroids are single-stranded RNA molecules; however, viroids are smaller in size and are not encapsidated. Potato spindle tuber viroid was the first viroid that was discovered in 1971. To date, twenty-nine more viroids have been discovered and characterized.
Studies indicate that the HpLVd is mainly introduced by infected propagation material. Subsequent viroid transmission from infected to healthy plants is shown to be through horticultural practices. Such mode of transmission (mechanical transmission) is also very common in phytopathogenic viral species. In hops, transmission of HpLVd was shown to be more effective with tools (e.g. pruning shears) rather than natural abrasions caused by physical contact of individual plants. In addition, no evidence of transmission and long-distance spread of the viroid by aphids has been presented.
The main component of successful management of HpLVd is prevention. Using disease-free seeds and propagation material is the most effective way of avoiding HpLVd. CSP Labs is equipped to test high volumes of seeds and plant material for HpLVd with high throughput molecular testing to ensure rapid turnaround times. Moreover, our plant pathologists have developed assays to test multiple diseases along with HpLVd simultaneously.
Throughout decades of active collaboration with seed companies, nurseries, and farmers of all sizes, CSP Labs has stayed focused on two principles; quality results and excellent customer service. In addition to disease testing on hemp, our genotyping lab provides variety identification and sex marker testing to the hemp industry”
More good Information:
What is Hop Latent Viroid in Cannabis - CTU
Hop latent viroid cannabis
cannabistraininguniversity.com
Everything Cannabis Growers need to know about Hops Latent Viroid - Green Leaf Lab
Viruses have had a profound effect on human history. Researchers work tirelessly every day uncovering how to better combat viruses that affect not only humans and animals, but plants as well. The Hop Latent Viroid (HpLVd), a single-stranded, circular infectious RNA, runs rampant in the hemp and...
greenleaflab.org
The molecular structure of hop latent viroid (HLV), a new viroid occurring worldwide in hops
Abstract. A new viroid which does not seem to produce any symptoms of disease, and is therefore tentatively named hop latent viroid (HLV) was found to occu
academic.oup.com
Cannabis Viruses, Viroids, and Phytoplasmas
What is a plant virus? A virus is an infectious nucleic acid-based pathogen that is parasitic to the host. Essentially, it is a non-host genome that ‘hijacks’ the replication machinery …
indicainfo.com
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Prevention Tools and sanitize prevention methods/protocols:
1. Treat your plants like a patient and you are the surgeon/doctor . Every cut and touch brings a possible issue. Key is proper cleanliness and steps to eliminate the worry of mass spreading and way higher % of healthy plants.
2. Use a true virusicide and something proven by the maker of the company that has been tested to eliminate HPLVD ( meaning in ur hands, surfaces of walkways, scissors)
3. My suggestion for product is physan 20, the company 100% has tested this and is not dangerous like bleach ( breath in and skin) , yes it will corrode your scissors if you let them sit in there 24/7. So my hint is flip em upside down after ur work and that will also remind you the physan 20 /virusicide is there ( don’t wanna add that to ur plants/misc). like many products its better to have the container or sprayer as used for only that product or use. labeling and having those protocols can change such a issue and also success everytime.
4. Key with using any proven product is knowing the time it takes to kill the virus/issue, bleech it would take minimum 20 minutes . Bleach isnt ag approved and also has many negative things for you and a garden, plus it does not have long timing defense since it evaporates quickly Physan is affordable but also very strong so I even clean my bathroom and misc with it without worrying of hurting myself but also having a longer term defense for most disease, virus and molds.
5. Physan 20 takes 10 minutes to fully clean tools of HPLVD , so what I do is have 3+ Scissors sitting in the physan water , i than keep using same scissors till I switch to new plant , before touching new plant I also spray my gloved hands with physan 20. having more than 3 scissors I haven’t yet have to “wait” for 10 minutes . Easy peezy and makes this protocol doable and safe!
Physan
Physan20 is a broad range disinfectant, fungicide, virucide, and algaecide which effectively controls a wide variety of pathogens on hard surfaces and plants. Its applications include greenhouses, hard surfaces, lawn and turfgrass, seedlings
physan 20 for hplvd cleaning rate 1.5T per gallon (highest rate, same rate as black mold), all tools or w/e ur protecting against such needs min 10 minutes before 100% clean
i noticed my keeepers and stock that I haven’t ever bought, don’t look in anyway like hplvd or dudd’s . But so many times I’ve seen others grow rooms or the store bought clones, kinda look infected (since looking more into it) to me compared to seeds germ and treated from start clones and Ipm for hplvd. Key is Inless you have serious cash is to never have it and treat this and many issues like a wild fire and think of the effects of the conqueneces if you let those kind of issues join in the garden
known labs that test seeds/plant material for HPLVD:
Node Labs
Node Labs based in the Bay Area is a state of the art micro tissue culture lab, cannabis clone cleaning and testing facility using proprietary processes and cutting-edge technology.
www.nodelabsca.com
Sample Collection Tube - TUMI Genomics
Tubes designed for the collection and stabilization of plant material infected with pathogens. (A minimum of 10 total tubes must be ordered)
tumigenomics.com
HpLVd - Hop Latent Viroid - CSP Labs
csplabs.com
Hops Latent Viroid Testing - HpLVd Cannabis Testing Lab
HpLVd can cause the plant to drop dramatically in efficacy. Cannabis infected with HpLVd may have half the cannabinoid content of their healthy counterparts. Call us for testing.
greenleaflab.org
